By Natasha S. Barteneva, Ivan A. Vorobjev
This distinctive quantity for the 1st time explores thoughts and protocols regarding quantitative imaging movement cytometry (IFC), which has revolutionized our skill to research cells, mobile clusters, and populations in a notable model. starting with an advent to expertise, the publication keeps with sections addressing protocols for experiences at the phone nucleus, nucleic acids, and FISH suggestions utilizing an IFC tool, immune reaction research and drug screening, IFC protocols for apoptosis and phone dying research, in addition to morphological research and the id of infrequent cells. Written for the hugely winning Methods in Molecular Biology sequence, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and tips about troubleshooting and warding off identified pitfalls.
Authoritative and useful, Imaging stream Cytometry: equipment and Protocols may be a severe resource for all laboratories trying to enforce IFC of their learn studies.