Applications of Infrared Spectroscopy in Biochemistry, by Frank Parker

By Frank Parker

This publication isn't meant to be a easy textual content in infrared spectroscopy. Many such books exist and i've said them within the textual content. particularly, i've got attempted to discover functions that may be attention-grabbing to various humans: complicated undergraduate chemistry scholars, graduate scholars and study employees in different disciplines, spectros­ copists, and physicians lively in examine or within the perform of drugs. With this objective in brain there has been no motive to have exhaustive assurance of the literature. I may still prefer to recognize my use of a number of books and stories, that have been useful in my look for fabric: G. H. Beaven, E. A. Johnson, H. A. Willis and R. G. 1. Miller, Molecular Spec­ troscopy, Heywood and corporate, Ltd., London, 1961. J. A. Schell guy and Charlotte Schellman, 'The Conformation of Polypeptide Chains in Proteins," within the Proteins, Vol. II, 2d Ed. (H. Neurath, ed.), educational Press, big apple, 1964. R. T. O'Connor, "Application of Infrared Spectrophotometry to Fatty Acid Derivatives," J. Am. Oil Chemists' Soc. 33, 1 (1956). F. L. Kauffman, "Infrared Spectroscopy of fat and Oils," J. Am. Oil Chemists' Soc. 41,4 (1964). W. J. Potts, Jr., Chemical Infrared Spectroscopy, Vol. I, concepts, Wiley, big apple, 1963. R. S. Tipson, Infrared Spectroscopy of Carbohydrates, nationwide Bureau of criteria Monograph llO, Washington, D.C., 1968. C. N. R. Rao, Chemical purposes of Infrared Spectroscopy, educational Press, manhattan, 1963.

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Chern. Soc. 1961, 2382, 3063, and references cited therein. Potts, W. , Jr. Chemical Infrared Spectroscopy, Vol. I: Techniques, Wiley, New York, 1963. Sober, H. , ed. Handbook of Biochemistry, Selected Data for Molecular Biology, The Chemical Rubber Company, Cleveland, Ohio, 1968. Spencer. B. Biochem. J. 73. 442 (1959). Szymanski, H. A. Interpreted Infrared Spectra, Vol. 2, Plenum Press Data Division, New York, 1966. Wilson, E. , Decius, J. , and Cross, P. C. Molecular Vibrations. McGraw-Hill, New York, 1955.

It shows positions, ranges, and types of characteristic bands [see also, Goddu (1960)]. The length of the lines indicates the normal range within which the bands are usually found. Most of the absorptions indicated in Fig. 611, are due to overtone bands. 27 Near-Infrared Spectroscopy Group 0·6 - CH3 ... ...... ...... ;CH2 ~CH ..... -CHArom. - CHAld. - NHz :NH - IVHzAmide ... - OH Alkyl .... - OHPheno/ -OH ACId ..... ":. ... ~ ... -... - DC Alk -FH ..... ~ SH CO 0-6 1·8 1·0 - ...... ---- -...

Trans-unsaturated and saturated C-H groups give no interference. Therefore, mixtures of cis, trans, and terminal double bonds can be analyzed readily for cis and terminal bonds (Kauffman, 1964). The stretching vibration ofthe OH group of mono glycerides has been examined in the region 7692-6667 cm -1 (Kauffman, 1964). 1-Mono- and 2-monoglycerides can be quantitated by plots of absorbances of the 6993 cm - 1 band versus concentration. The C-H stretching bands of 1,2- and 1,3-diglycerides have been investigated and the spectra of these isomers showed possibilities of three main configurations (Kauffman, 1964), one internally hydrogen-bonded, and two with no hydrogen bond involvement.

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